Comparative Neuromuscular Laboratory

Sample Requirements

Requirements for samples submitted to Comparative Neuromuscular Lab
Test Sample Requirement Cost Turnaround Time
Complete Muscle Profile (900)

A biopsy approximately 0.5 X 0.5 X 1.0 cm should be taken from an affected but not end-stage muscle by an open biopsy procedure. Wrap the muscle specimen in a saline dampened (not dripping, just moist) gauze sponge, place into a dry water tight container (5 or 10 ml red top tube, dry urine cup) and keep refrigerated until shipped to the laboratory on cold packs. Collect a second smaller biopsy and immersion fix in 10% neutral buffered formalin. For optimal results the specimens need to stay chilled and be received by the laboratory within 24-48 hours of collection.

Useful Link: Complete Muscle Profile

Useful reference: Muscle and Nerve Biopsy. Vet Clinics North America (2002) 32: 63-102.

$159.00 5-7 working days
Each additional muscle (901) Additional charges apply if biopsies are submitted from more than 1 muscle specimen. $84.00 5-7 working days
Peripheral nerve profile ONLY (first nerve) (902)

The Peripheral Nerve Profile includes evaluation of resin embedded 1um thick sections for determination of axonal degeneration, demyelination, and nerve regeneration. The fixed nerve specimens can be further processed for ultrastructural analysis and teased nerve fibers on a case-by-case basis at additional charge. Please call for charges. Frozen nerve biopsy sections are used for immunohistochemical analysis on a case-by-case basis. A biopsy specimen approximately 2.5 cm (1 inch) in length should be divided with 2/3 placed on a tongue depressor to maintain length (do not stretch) and immersion fixed in 10% neutral buffered formalin. 1/3 of the biopsy specimen should also be placed on a tongue depressor, wrapped in a saline dampened gauze sponge, placed into a water tight container, and kept chilled during shipping. Peripheral nerve biopsy should only be performed by a veterinarian experienced in this procedure.

Useful reference: Muscle and Nerve Biopsy. Vet Clinics North America (2002) 32: 63-102.

$159.00 5-7 working days
Each additional nerve (901) Additional charges apply if biopsies are submitted from more than 1 peripheral nerve specimen. $84.00 5-7 working days
Combined muscle and peripheral nerve profile(903) A special rate applies to a single muscle and peripheral nerve biopsy submitted together from the same case. Additional fees will apply if multiple muscles and nerves are submitted (901). $249.00 5-7 working days
2M antibody (Masticatory Muscle Myositis) (904)

Submit 1-2 ml of serum in a red top tube via standard overnight or second day service. Since this is an antibody based test, collect the serum sample PRIOR to initiation of corticosteroid or other immunosuppressive therapy.

Useful Link: Myositis

$141.00 5-7 working days
Acetylcholine receptor antibody (Myasthenia Gravis) (905)

Submit 1-2 ml of serum in a red top tube via standard overnight or second day service. Since this is an antibody based test, collect the serum sample PRIOR to initiation of corticosteroid or other immunosuppressive therapy.

Useful Link: Myasthenia Gravis

$141.00 5-7 working days
Special Immunohistochemistry (907) Immunohistochemical testing procedures are performed on fresh frozen muscle biopsy sections, usually following the Complete Muscle Profile. Individual tests can include selected staining from the dystrophy panel (907.1) selected muscle proteins or staining for CD8+ T cells/MHC Class I to support a diagnosis of polymyositis. $111.00 14-21 working days
Dystrophy panel (907.1)

This is an expanded immunohistochemistry panel for detection of many proteins that result in muscular dystrophy including but not limited to dystrophin, sarcoglycans, laminin a2, dysferlin, a- and b- dystroglycans, utrophin and spectrin.

Useful link: Representative dystrophy panel showing stainings from a dog with dystrophin deficient muscular dystrophy.

Useful reference: Routine and specialized laboratory testing for the diagnosis of neuromuscular diseases in dogs and cats (2010) 39: 278-295.

$371.00 14-21 working days
Dystrophy immunoblot (907.2) n extracts from submitted muscle biopsies are probed with various monoclonal and polyclonal antibodies against dystrophy associated proteins to detect an absence, abnormality in size or amount. This testing procedure further confirms the results of the dystrophy panel by immunohistochemistry. $276.00 14-21 working days
Myositis Panel (907.3) This panel is useful in characterizing cellular infiltrates in frozen muscle biopsy specimens from cases of myositis. Determination of B cell, T cell, and macrophage populations may aid in choosing specific therapies. In addition, up-regulation of Major Histocompability (MHC) antigens on muscle membranes can aid in the diagnosis of immune-mediated polymyositis when only minimal cellular infiltrations are present in the biopsy specimen. $371.00 14-21 working days
Sarcolemmal Antibody (907.4)

Sarcolemmal staining of muscle fibers using specific immunoreagents can be found in cases of immune-mediated myositis. Antibodies can be detected in the serum (2ml shipped by an overnight service) or directly within frozen muscle biopsy specimens.

Useful reference: Vet Immunol Immunopath 2006:116;113;1-10.

$111.00 14-21 working days
Antistrial Antibody (907.5)

Antibodies against muscle striational proteins can have diagnostic usefulness in cases with a cranial mediastinal mass and paraneoplastic associated muscle weakness from either myasthenia gravis or polymyositis. Identification of antistrial antibodies are thymoma associated. This serum test requires 2 ml of serum shipped by an overnight service.

Useful Link: What are antistrial antibodies and when is testing indicated?

$111.00 14-21 working days
DNA purification and storage Submit 5 ml of EDTA whole blood packaged with a cold pack and shipped by an overnight or second day service such as FedEx or UPS. DO NOT USE USPS. Ship DNA samples between Monday and Wednesday. $91.00 Call lab 858-534-1537